PDF | Ethnopharmacological relevance: Jatropha gossypiifolia L. ( Euphorbiaceae) is popularly known as bellyache Download full-text PDF. Download scientific diagram | Jatropha gossypifolia (fam. Euphorbiaceae) no município de Seropédica, RJ. from publication: Intoxicação experimental com as . Jatropha gossypiifolia L. (Euphorbiaceae), widely known as “bellyache bush,” biological activities such as Jatropha curcas, Jatropha elliptica.

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The medicinal tropic plant Jatropha gossypifolia L. fx1. Download high-res image (KB) · Download full-size image. Previous article in issue. Biochemical Systematics and Ecology 27 () } A rare diterpene from Jatropha gossypifolia Biswanath Das*, B. Venkataiah Organic Chemistry. Purpose: To evaluate α-glucosidase and α-chymotrypsin enzyme inhibitory activity of Jatropha gossypifolia as a probable remedy for the management of.

A rare diterpene from Jatropha gossypifolia

During every experiment, mice were divided into four groups each of containing 5 mice. For further reference, a duplicate voucher specimen Accession No. DACB of the plant has been deposited. Preparation of methanol extract The collected fruits were washed and sun dried for 7 days. The dried fruits were ground to coarse powder with the help of a mechanical grinder and were stored in an airtight glass container.

The powdered plant material g was extracted at room temperature by maceration 1. Experimental animals Swiss albino mice of either sex were downloadd from the Animal Research Branch of the International Center for Diarrheal Disease and Research, Bangladesh were taken for the tests.


Mice were housed under standard laboratory conditions room temperature Analgesic activity The analgesic activity of the extract was evaluated using acetic acid induced writhing method in mice. Each group received a particular treatment, i. After 30 min of oral administration of control and test groups, 0.

The positive control was administered orally 15 min prior to acetic acid injection.

Full writhing was not always accomplished by the animal, because sometimes the animals started to give writhing but they did not complete it. This incomplete writhing was considered as half-writhing. Accordingly, two half-writhing were taken as one full writhing.

Each mouse was immediately placed on one side of the specified instrument, after oral administration of test drugs. The spontaneous movement of the mice from one chamber to other through the hole was observed for 3 min.

The observation was conducted at 0, 30, 60, 90, and min. Hole-board test The method described by Somani et al. The study was conducted using a wooden hole-board apparatus measuring 20 cm by 40 cm with 16 evenly spaced holes each of diameter 3 cm.

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The apparatus was elevated to a height of 25 cm. Combination therapy is effective in early stages of infection but becomes ineffective after prolonged exposure Coulon et al.

The high failure rate of medication may be partially due to poor absorption of topical anti-amoebic drugs by the thickened sclera Seal, or ineffectiveness of these drugs in killing the highly resistant cysts and recurrence after stopping of treatment Coulon et al.

Plant products could be used for Acanthamoeba infection treatment as they are rich sources of bioactive metabolites Kayser et al.

A rare diterpene from Jatropha gossypifolia

Nanoparticles are elementary structures of nanotechnology and are important materials for fundamental studies and various applications, including their bioactivities Patil et al. Phytosynthesized silver nanoparticles AgNPs as an effective biological agent have potential over physicochemical modes of synthesis because of their ecosafety and unique synthesis mechanism Patil et al. The plants Jatropha curcas, Jatropha gossypifolia and Euphorbia milii used in the present study have been reported to have medicinal application as well as active chemical constituents Leitsch et al.

Jatropha curcas, J. Amoebicidal and cytotoxic capacity of the AgNPs synthesized from Euphorbian plant extracts is reported here. Materials and methods Preparation of plant extracts Fresh leaves of plants under study J.

Taxonomic identification was done by L. Leaves were dried and finely ground to powder. During incubation, the color of the solution changed to yellowish brown, indicating the formation of AgNPs. This brown solution was used for screening of amoebicidal activity and further characterization of AgNPs by UV-Vis spectrophotometry, scanning electron microscopy SEM , particle size and zeta potential analysis.

For the experiment, 1 mL of the culture was centrifuged for 5 min at g, and the pellet washed twice with phosphate-buffered saline PBS. Amoebicidal assay For the assessment of amoebicidal activity, a 0.

Viability was assessed using methylene blue. Test concentrations of 50, 25, Cytotoxicity assay in peripheral blood mononuclear cells Working stock of AgNPs was prepared, and 0.

After incubation, cell viability was determined by 4,5-dimethylthiazolyl -2,5-diphenyltetrazolium bromide assay Sigma, St.The dried fruits were ground to coarse powder with the help of a mechanical grinder and were stored in an airtight glass container. Inhibition of edematogenic activity The edematogenic activity of B.

After becoming solidified, holes of 0. Additionally, the fibrinogenolytic activity of B.

Analgesic activity The analgesic activity of the extract was evaluated using acetic acid induced writhing method in mice. Prothrombin Time PT test The action in extrinsic pathway was evaluated by PT test, as previously described in literature, with a few modifications[ 24 ].

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